2019/10/18最新更新专利技术
  • 一种新型链霉菌胰蛋白酶GM2938及其异源表达的制作方法
    本发明涉及一种链霉菌胰蛋白酶GM2938的异源表达及其应用的。链霉菌胰蛋白酶是微生物胰蛋白酶家族中的重要一员,因其具有较好的生物安全性、生产品质可控性、非钙离子活化、与哺乳动物牛胰蛋白酶存在同源性且酶学性质也与牛胰蛋白酶相似等优点,而被广泛的应用到制药、临床诊断、食品加工、皮革加工...
  • 碱性蛋白酶PA3及其编码基因和应用的制作方法
    本发明属于农业生物,具体涉及碱性蛋白酶PA3及其编码基因和应用。蛋白酶指通过切割肽键实现蛋白质水解的一类酶,其来源十分广泛,大量分布于动物、植物、微生物中。相比于动植物来源的蛋白酶,微生物源蛋白酶具有培养方便、操作简单和产酶量高等便于工业化的批量生产的特点,因此,微生物源蛋白酶成为...
  • 一种具有高分泌能力的普鲁兰酶突变体及其应用的制作方法
    本发明涉及一种具有高分泌能力的普鲁兰酶突变体及其应用,属于酶工程和微生物工程。淀粉是植物体中贮存的养分,主要贮存在植物种子和块茎中,储量十分丰富。除食用外,淀粉主要被用于糊精、麦芽糖、葡萄糖、酒精等产品的制备,应用十分广泛。在利用淀粉制备糊精、麦芽糖、葡萄糖、酒精等产品的过程中,高...
  • 一种可提高葡萄酒风味与品质的酶制剂的生产与应用方法与流程
    本发明涉及工业微生物,具体是一种可提高葡萄酒风味与品质的酶制剂的生产与应用方法。葡萄酒是指以鲜葡萄或葡萄汁为原料,经全部或部分发酵酿制而成的,含有一定酒精度的发酵酒。香气是葡萄酒最重要的品质,葡萄酒香气主要可分为三大类,分别为来源于葡萄浆果本身的品种香气、葡萄酒酿造过程中产生的发酵...
  • 一种利用里氏木霉分批补料发酵高效生产β-葡萄糖苷酶的方法与流程
    本发明属于微生物发酵,具体涉及一种利用里氏木霉分批补料发酵高效生产β-葡萄糖苷酶的方法。β-葡萄糖苷酶又称β-D-葡萄糖苷水解酶,该酶可以将结合于原子基团末端的非还原性糖苷键水解,从而释放葡萄糖和配基分子。因此,该酶可以应用于生物能源领域,配合纤维素酶将木质纤维素彻底水解为葡萄糖用...
  • 一种梨果实液泡酸性转化酶及其活性抑制因子的应用的制作方法
    本发明涉及一种梨果实液泡酸性转化酶及其活性抑制因子的应用。转化酶(invertase,INV)是一种水解酶,不可逆地催化蔗糖转化为果糖和葡萄糖,其不仅在初级碳代谢中发挥功能,还广泛参与植物生长发育的多个调节过程。根据最适pH的差异,转化酶可分为酸性转化酶和中性/碱性转化酶两大类。其中,酸性...
  • 一种耐高温α-鼠李糖苷酶基因及其转化芦丁生成异槲皮素方面的应用的制作方法
    本发明属于基因工程技术及生物医药领域,具体涉及一种α-鼠李糖苷酶及其转化芦丁制备异槲皮素方面的应用。芦丁是一种常见的黄酮类化合物,广泛存在于中草药、水果、蔬菜以及许多植物衍生中。它具有抗氧化,抗高血糖和神经?;ぷ饔玫榷嘀稚锘钚?。研究表明,芦丁与它的去鼠李糖基产物异槲皮素(槲皮素-3-β-...
  • 用于检测有机磷农药的突变酶及制备方法与流程
    本发明属于生物,尤其涉及一种检测有机磷农药的突变酶及其制备方法。有机磷农药因具有效率高、品种多、用途广等特点而被广泛使用。有机磷农药的使用,对农业生产上作物?;て鹆司薮蟮淖饔?,但同时,由于有机磷不易氧化、分解,也难被微生物降解,能长期在环境中滞留,因此,有机磷农药的大量使用,造成了...
  • 合成呋喃酮及其衍生物葡萄糖苷的糖基转移酶突变体蛋白的制作方法
    本发明属于基因工程领域,具体涉及一种合成呋喃酮及其衍生物葡萄糖苷的糖基转移酶突变体蛋白。呋喃酮(4-羟基-2,5-二甲基-3(2H)-呋喃酮,Furaneol,DMHF),又称菠萝酮或草莓酮,分子量为128.13,是一种白色至浅黄色结晶体或粉末状固体。熔点为78-80℃,微溶于水,易溶于乙...
  • 一种金银花查尔酮合酶突变体及其用途的制作方法
    本发明属于生物,特别涉及一种金银花查尔酮合酶突变体及其用途。金银花(FlosLoniceraeJaponicae)为忍冬科(Caprifoliaceae)忍冬属(Lonicera)植物忍冬(Lonicera.japonicaThunb.)的干燥花蕾,忍冬属植物在全世界约有20...
  • 一种谷氨酰胺转氨酶发酵液的连续脱色方法与流程
    本发明涉及一种谷氨酰胺转氨酶发酵液的连续脱色方法。谷氨酰胺转胺酶,是一种催化?;品从Φ淖泼?,它能够促使蛋白质分子内交联,蛋白质分子间交联以及蛋白质和氨基酸之间的交联,可以在很大程度上改善蛋白质的功能性质,提高蛋白质的营养价值,在食品加工业中有着广泛的应用。谷氨酰胺转胺酶最早在豚鼠肝脏...
  • 一种珠子参糖基转移酶UGTPjm1基因及在制备人参皂苷Ro上的应用的制作方法
    本发明属于生物,具体涉及一种珠子参糖基转移酶UGTPjm1基因及在制备人参皂苷Ro上的应用。珠子参(Panaxjaponicusvar.major)为五加科(Araliaceae)人参属(Panax)药用植物,以根状茎入药。目前已从珠子参的根状茎中分离鉴定出多种三萜皂苷,其含量...
  • 本发明涉及一种提高毕赤酵母过氧化氢酶表达量的方法,属于微生物领域。堆肥好氧发酵的生物化学过程都是在酶参与下进行的酶促反应。在堆肥腐熟期,过氧化氢酶、脱氢酶、多酚氧化酶等氧化还原酶发挥了作用,把有机物降解并进一步转化成腐殖质,实现了堆肥的无害化处理。过氧化氢酶是一种稳定的除氧酶,其作用是高效...
  • 一种L-谷氨酸脱氢酶突变体及其应用的制作方法
    本发明属于生物,具体涉及一种L-谷氨酸脱氢酶突变体及其应用。草铵膦是由赫斯特公司80年代开发的广谱触杀型除草剂。目前世界上的三大除草剂是草甘膦、草铵膦、百草枯,相对于草甘膦和百草枯,草铵膦具有优异的除草性能及较小的副作用。草铵膦有两种光学异构体,分别为D-草铵膦和L-草铵膦,但是只...
  • CYP52A12基因的定向进化及其在二元酸生产中的应用的制作方法
    本发明涉及利用定向进化和同源重组方法制备长链二元酸菌株的方法、利用该方法所获得的可以在酸性条件下生产长链二元酸的菌株及所述菌株的用途。具体而言,本发明涉及通过定向进化CYP52A12基因和同源重组方法制备长链二元酸菌株的方法、利用该方法所获得的可以在酸性条件下生产长链二元酸的菌株及所述菌株的用途。...
  • 一种利用固定化漆酶催化降解异黄酮的方法与流程
    本发明涉及一种利用固定化漆酶催化降解异黄酮的方法,属于生物工程领域。以来源于一色齿毛菌(Cerrenaunicolor)HYB07菌株的漆酶为对象,采用丙烯?;男源判晕⑶騀e3O4-SiO2-MPS为载体对漆酶进行固定化,利用固定化漆酶催化降解异黄酮化合物。漆酶作为高效多酚氧化酶,可作...
  • 用于聚合酶链式反应(PCR)的新型组合物、方法和试剂盒与流程
    本公开内容总地来说涉及用于扩增核酸同时减少非特异性扩增和/或非期望的扩增产物的组合物、方法和试剂盒。虽然聚合酶链式反应(PCR)和相关技术对于各种应用是高度有用的,但因非期望的副反应而产生的非靶核酸的扩增可呈现严重问题。这样的副反应可因非靶核酸的错误引发(mis-primin...
  • 一种抗尿苷酸反馈抑制的氨甲酰磷酸合成酶突变体及其应用的制作方法
    本发明涉及酶工程,尤其涉及一种抗尿苷酸(UMP)的氨甲酰磷酸合成酶突变体及其应用。氨甲酰磷酸合成酶(CPS)分为两种CPSⅠ和CPSⅡ,两者为同工酶,CPSⅠ是参与肝脏细胞能量代谢中尿素循环中的关键酶,可以去除细胞中多余的氨,此酶的缺失会导致高氨血症,CPSⅡ(EC6.3.5.5...
  • 一种表达VP2基因的重组Ⅰ型马立克氏病病毒疫苗毒株的制作方法
    本发明属于分子生物学和基因工程疫苗,具体涉及一种表达传染性法氏囊病毒VP2基因的重组马立克氏病毒株,该重组病毒免疫家禽可用于传染性法氏囊病及马立克氏病的预防。技术背景马立克氏病(Marek’sdisease,MD)和鸡传染性法氏囊病(Infectiousbursaldisease,I...
  • 本发明涉及一种表达猪圆环病毒Cap蛋白基因的重组猪伪狂犬病病毒弱毒株、制备方法及利用该重组病毒株制备的疫苗组合物,属于兽用生物制品领域。猪圆环病毒(Porcinecircovirus,PCV)属于圆环病毒科圆环病毒属,是目前为止发现的最小的脊椎动物病毒。PCV根据致病性、抗原性及核苷酸序...
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