2019/10/18最新更新专利技术
  • 四种阴道泌尿道支原体多重检测的引物、探针组、试剂盒及检测方法与流程
    本发明涉及病原微生物的检测,尤其涉及四种阴道泌尿道支原体多重检测的引物、探针组、试剂盒及检测方法。支原体(mycoplasma)是一类没有细胞壁、高度多形性、能通过滤菌器、可用人工培养基培养增殖的最小原核细胞型微生物,大小为0.1~0.3微米。支原体主要以二分裂方式繁殖,亦可以出芽...
  • 近海水域中大肠杆菌的快速检测方法与流程
    本发明涉及常见病原菌检测,涉及到大肠杆菌保守性基因片段的一种q-PCR检测方法。近岸海域是人类赖以生存的主要环境区域之一,该海域受人类活动影响较大,生态系统非常脆弱。近年来随着区域经济的快速发展,近岸海域所承受的环境压力不断加大,海洋赤潮等生态灾害频发,生态环境趋于恶劣。更有甚者随...
  • 双重检测加德纳杆菌和阴道毛滴虫的引物、探针组、试剂盒及检测方法与流程
    本发明涉及病原微生物的检测,尤其涉及双重检测加德纳杆菌和阴道毛滴虫的引物、探针组、试剂盒及检测方法。阴道炎(vaginitis)即阴道炎症,是导致外阴阴道症状如瘙痒、灼痛、刺激和异常流液的一组病症。正常情况下有需氧菌及厌氧菌寄居在阴道内,形成正常的阴道菌群。任何原因将阴道与菌群之间...
  • 一种鉴别贵州地方猪种与大白猪的KLF8基因中SV103分子标记及其检测技术的制作方法
    本发明涉及动物分子生物学检测领域,具体来说,涉及一种区分香猪和大白猪品种的分子标记和检测技术。香猪是我国特有的小型猪种,早在20世纪70年代末被发现于贵州省都柳江与广西接壤区域的山区,主产区位于贵州省从江县宰便、加鸠两地。从江香猪是我国贵州省从江县特殊的生态环境下,经自然选择和人工选育形成...
  • 一种用于鉴定香猪品种的结构变异TRO基因中SV108分子标记及其检测技术的制作方法
    本发明涉及动物分子生物学检测领域,具体来说,涉及一种用于鉴定香猪品种的结构变异TRO基因中SV108分子标记及其检测技术。香猪长期在密闭的森林里繁衍生息,逐渐形成近交不退化,基因高度重合的小型猪种,产区分散居住着苗、壮、瑶、水等少数民族,耕地较少,交通不便,农作物以糯谷和粳稻为主,这里生活...
  • 一种鉴别香猪与大白猪的结构变异SMC1A基因中SV320分子标记及其检测技术的制作方法
    本发明涉及动物分子生物学检测领域,具体来说,涉及一种区分香猪和大白猪品种的分子标记和检测技术。香猪是我国特有的小型猪种,早在20世纪70年代末被发现于贵州省都柳江与广西接壤区域的山区,主产区位于贵州省从江县宰便、加鸠两地。香猪屠宰时,二月龄断奶的乳猪无乳腥气味;出栏肥猪宰杀开膛,腹腔无腥臭...
  • 一种用于鉴定中国地方猪种香猪的结构变异KLF8基因中SV322分子标记及其检测技术的制作方法
    本发明涉及动物分子生物学检测领域,具体来说,涉及一种用于鉴定中国地方猪种香猪的结构变异KLF8基因中SV322分子标记及其检测技术。香猪主要以放牧为主,85%以上为粗饲料,生产地多为山青水秀,环境优美,无任何工业污染,加之生长周期短,个体小,所以香猪肉嫩味香,回味深长,素有“一家煮肉四邻香...
  • 一种用于11个物种种属来源鉴别的PCR-STR检测试剂盒的制作方法
    本发明属于法医遗传学领域,具体涉及公开一种可同时用于人、鸡、鸭、羊、猪、牛、马、狗、大鼠、小鼠、鸽子共11个物种种属来源鉴别的短串联重复序列基因座试剂盒及其分型检测方法,其特别适合法医种属来源鉴定和食品安全领域肉类食品种属来源的检测和甄别。肉类及肉制品是人类日常餐食中的重要组成部分,也是人...
  • 一种用于花生蚜虫PCR检测的COI引物及其应用的制作方法
    本发明涉及农业,具体涉及一种用于花生蚜虫PCR检测的COI引物及其应用?;ㄉ谴笞谟土献魑?,在中国有着广泛的种植,同时我国也是花生第一大消费国和出口国?;ㄉ脸媸俏ㄉ闹匾Τ嬷?,该种害虫广泛分布于我国各花生主产区,通常以成、若蚜群集在花生的嫩芽、嫩叶以及果针等处为害,常导致...
  • 鸡ANXA2基因5′调控区三个突变位点分子标记方法及其在鸡育种中的应用与流程
    本发明涉及分子遗传学,具体涉及一种鸡ANXA2基因5′调控区三个突变位点分子标记方法及其在鸡育种中的应用。膜联蛋白(Annexins)是一种多基因的依赖钙离子的磷脂结合蛋白超家族。AnnexinA2(p36)是AnnexinsA亚族的成员,是一种多功能蛋白,和该家族的其他成员一...
  • 凡纳滨对虾微卫星分子标记特异性引物及其在遗传多样性分析中的应用的制作方法
    本发明属于分子生物学DNA分子标记,尤其是一种凡纳滨对虾微卫星分子标记特异性引物及其在遗传多样性分析中的应用。凡纳滨对虾(Litopenaeusvannamei),又名南美白对虾、万氏对虾、白对虾,隶属于对虾科(Penaeidae)、对虾属(Penaeus),原产于中、南美洲...
  • 一种基于QPCR定量检测畜禽肉中牛源性成分的方法与流程
    本发明属于分子生物学分析领域,具体涉及肉制品中牛源性成分含量的鉴定方法,是一种在实时荧光定量PCR技术基础上利用内参基因校正外源种属特异性基因对牛源性成分进行定量检测的方法。多源化的肉类品种是人们餐桌文化日趋丰富的重要组成部分,然而随着市场上牛肉价格的不断大幅上涨,部分不法企业及商贩,为了...
  • 一种用于贲门癌高危人群筛查的SNP标志物及试剂盒的制作方法
    本发明属于基因工程及肿瘤医学领域,具体涉及一种用于贲门癌高危人群筛查诊断的SNP标志物及试剂盒。贲门是位于食管胃交界部,齿状线下方2cm以内的组织器官。由于贲门解剖范围较小,该部位发生癌变后组织侵犯范围较广,较难准确定位肿瘤的部位,因此国际上对发生于食管下段胃交界部的肿瘤统称为食管胃交界部...
  • 检测结直肠癌相关基因甲基化的检测试剂的应用和试剂盒的制作方法
    本发明涉及基因检测,具体而言,涉及检测结直肠癌相关基因甲基化的检测试剂的应用和试剂盒。结直肠癌是世界第三大恶性肿瘤。中国近年来结直肠癌发病率逐年上升,估计每年约有40万新发病例,在我国消化系统恶性肿瘤中位列第二。结直肠癌通常由癌前息肉经过一系列遗传学和表观遗传学改变及组织学与形态学...
  • 一种人类微卫星不稳定性状态MSI检测位点及检测方法和应用与流程
    本发明涉及人类微卫星不稳定性状态MSI的检测,具体涉及人类微卫星不稳定性状态MSI检测位点的筛选,利用MSI-PCR实验方法检测结直肠癌患者的MSI状态。微卫星(microsatellite)是指DNA基因组中小于10个核苷酸的简单重复序列,遍布于整个人类基因组,一般为2-6个碱基重复。这...
  • 膀胱癌标志物组及其应用的制作方法
    本发明属于分子生物学领域。具体地,本发明涉及膀胱癌标志物组及其应用。更具体地,本发明涉及膀胱癌的DNA甲基化生物标志物及其应用。随着人们对肿瘤认识的加深以及科学技术的进步,许多新型的肿瘤标记物被发现并用于临床诊断。1980年之前,肿瘤标记物主要是一些激素、酶类、蛋白质等细胞分泌物,例如癌胚...
  • 前列腺癌的标志物组及其应用的制作方法
    本发明属于生物医学领域。具体地,本发明涉及前列腺癌的标志物组及其应用。在世界范围内,前列腺癌发病率位居男性恶性肿瘤第2位。近30年来前列腺癌发病率在大部分国家都有明显的升高,目前前列腺癌在美国的发病率已经超过肺癌,在危害男性健康的肿瘤中排第一。我国前列腺癌发病率较低,但近年来呈显著上升趋势...
  • Sox30基因在制备检测和治疗卵巢癌药物中的应用的制作方法
    本发明涉及卵巢癌治疗,具体涉及Sox30基因在制备检测和治疗卵巢癌药物中的应用。在全球范围内,卵巢癌死亡率一直高居女性生殖系统恶性肿瘤首位,因其解剖位置特殊,深藏于盆腔,早期无明显症状,70%以上发现时已为晚期,其病死率高,5年生存率仅为40%~45%。发达国家的卵巢癌发病率为9....
  • TRPV4的用途及其抑制剂的用途和药物筛选方法与流程
    本发明涉及抗癌,特别涉及一种TRPV4的用途及其抑制剂的用途和药物筛选方法。肠道癌症是最常见的癌症类型之一,包括结肠癌和直肠癌等。最新调查数据显示,所有恶性肿瘤中,结直肠癌死亡率为全球第二,发病率为全球第三。肠道癌症的发生与高脂肪低纤维素饮食、肠道慢性炎症、遗传因素和其他因素(如环...
  • 基于转录学的黄芪散精准干预高脂血症生物标志物检测方法与流程
    本发明涉及药物,特别涉及一种基于转录学的黄芪散精准干预高脂血症生物标志物检测方法。高脂血症(Hyperlipidemia)是一种脂质代谢紊乱性疾病,以血液中甘油三酯(TG)、胆固醇(TC)、低密度脂蛋白升高(LDL-C)和高密度脂蛋白(HDL-C)降低为主要特征,能够引起动脉硬化、...
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